Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

New Protocols From Expert Users to Enable Enhanced Protein Analysis
Reviews

From the reviews:

"It is no surprise that a book of this title is somewhat eclectic. ... Methodologies are well written and useful, emphasizing that proteins and function can be studied at differing levels of resolution. ... this book will be useful for beginners, though it is really more for the initiated seeking inspiration." (Dr. Mike J. Naldrett, Microbiology Today, November, 2006)

"New and Emerging Proteomics Techniques contains detailed descriptions of modern proteomics techniques written by experts in the field. ... Overall, the book's background information and detailed protocols and notes would be of interest to researchers in many areas who are interested in knowing more about the types of studies now made possible by these powerful, combined or individual proteomics techniques, and how they might be applied to current questions in basic and medical science." (Constance J. Jeffery, Journal of the American Society for Mass Spectrometry, Vol. 18, 2007)

Book Information
ISBN 9781588295194
Author Dobrin Nedelkov
Format Hardback
Page Count 248
Imprint Humana Press Inc.
Publisher Humana Press Inc.