Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful.

Now including pathogens of additional invertebrates, the 2nd edition provides essential coverage of step-by-step protocols for the isolation, identification, propagation, bioassay and preservation of entomopathogens.

About the Author
Dr. Lacey earned his PhD in 1978 and has been internationally active working in medical entomology, insect pathology, and microbial control of economically and medically important insects. He conducted research on the biology and microbial control of dozens of insects in the US, Amazon Basin, West Africa in the Azores, throughout Europe, the Middle East and Western and Southeast Asia and the Pacific Northwest of the USA. In his 40 year career he has written or co-authored nearly 150 refereed papers in medical entomology, agricultural entomology, and insect pathology, and edited and co-edited seven books, including the Manual of Techniques in Invertebrate Pathology, second edition and Microbial Control of Insect and Mite Pests: From Theory to Practice.

Reviews
listing in Society of Invertebrate Pathology Newsletter



Book Information
ISBN 9780123868992
Author Lawrence A. Lacey
Format Paperback
Page Count 504
Imprint Academic Press Inc
Publisher Elsevier Science Publishing Co Inc
Weight(grams) 1280g