In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
Reviews
From Reviews of the First Edition...

"...will benefit the apprentices...and the experienced scientist venturing into a new technique or changing yeast species...should join the other 'cookery' books on the laboratory shelf." -Journal of Emergency Medicine

"...a useful addition to life sciences libraries and to laboratories embarking on studies with yeast"
-Quarterly Review of Biology



Book Information
ISBN 9781617375699
Author Wei Xiao
Format Paperback
Page Count 392
Imprint Humana Press Inc.
Publisher Humana Press Inc.