This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough,
Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field.
Book InformationISBN 9781071622353
Author Christopher R. ReischFormat Paperback
Page Count 264
Imprint Springer-Verlag New York Inc.Publisher Springer-Verlag New York Inc.