This book is the final part of a trilogy of volumes covering the practical aspects of ligand interactions, biochemistry, and effector mechanisms of signal-transducing receptors. The book focuses on the measurement of receptor binding interactions both at equilibrium and from the kinetic point of view. In addition to descriptions of the most important techniques for the performance of ligand binding assays to both membrane-bound and solubilized receptors. A systematic effort has been made to provide insight into the strategy and tactics of receptor binding assays, and the conditions which must be satisfied if artifacts are to be avoided and interpretable data obtained. Since the behaviuor of even the simplest receptor systems can be surprisingly complex, an attempt has been made to provide some computational tools which allow complications to be predicted and handled or avoided. Ligand binding assays are very widely used, not least in drug screening and development. This volume will help the practitioner to choose an appropriate ligand, make a suitable receptor preparation, design and perform a well thought-out set of assays, and analyse the data obtained correctly.
Reviews
'This is an excellent multi-author book. The book represents very good value for the amount of information that it contains and will appeal to both the experienced and those starting receptor research. Selected chapters would be of relevance to undergraduates embarking on final year projects.' Anthony P. Davenport and Peter Molenaar, Addenbrookes Hospital, Trends in Biochemical Sciences, Volume 17 No. 12, December 1992
'a very timely publication ... This book, together with Receptor Biochemistry and Receptor-Effector Coupling, the other two books in this series, would form an invaluable resource for receptor studies.' A. Sivaprasadarao, Biomedical Education 21 (1) 1993
'a valuable bench manual for all researchers in biochemistry, molecular biology, neuroscience, and pharmacology who are interested in receptor function' Anticancer REsearch 12: (1992)



Book Information
ISBN 9780199630912
Author E. C. Hulme
Format Paperback
Page Count 480
Imprint Oxford University Press
Publisher Oxford University Press
Weight(grams) 734g
Dimensions(mm) 235mm * 158mm * 26mm