A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes. In Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens. These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Reviews

From the reviews:

"This volume ... describes newly developed or optimised techniques for understanding the immune response of plants and the ways in which pathogens, including the true fungi, oomycetes, nematodes and bacteria, are able to subvert it. ... Where appropriate, methods are well illustrated with diagrams, photographs of equipment and expected results. In summary, this is a timely addition to this series and will be of immediate interest and use to all those working in this field." (Andrew James, Annals of Botany, Vol. 111 (1), January, 2013)

Book Information
ISBN 9781493957552
Author John M. McDowell
Format Paperback
Page Count 295
Imprint Humana Press Inc.
Publisher Humana Press Inc.