Recently Viewed

New

Expression Systems: Methods Express Michael Dyson 9781904842439

No reviews yet Write a Review
RRP: £40.00
Booksplease Price: £39.80
Booksplease saves you 1%

  Bookmarks: Included free with every order
  Delivery: We ship to over 200 countries from the UK
  Range: Millions of books available
  Reviews: Booksplease rated "Excellent" on Trustpilot

  FREE UK DELIVERY: When You Buy 3 or More Books - Use code: FREEUKDELIVERY in your cart!

SKU:
9781904842439
MPN:
9781904842439
Available from Booksplease!
Global delivery available
Global delivery available
Global delivery available
Global delivery available
Global delivery available
Availability: Usually dispatched within 5 working days

Frequently Bought Together:

Total: Inc. VAT
Total: Ex. VAT

Description

Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when.
Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following:

  • Baculovirus expression vectors
  • CHO cells
  • E. coli
  • HEK293-EBNA1 cells
  • Lactococcus lactis and other gram positive bacteria
  • S. cerevisiae
  • Transfected insect cells
  • Pichia pastoris
  • Mammalian cells using BacMam viruses
  • Lentiviral vectors
  • Wheat germ cell-free system

The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.
Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench.
In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.



Book Information
ISBN 9781904842439
Author Michael Dyson
Format Paperback
Page Count 288
Imprint Scion Publishing Ltd
Publisher Scion Publishing Ltd
Weight(grams) 690g
Dimensions(mm) 243mm * 173mm * 16mm

Reviews

No reviews yet Write a Review

Booksplease  Reviews