Molecular Biology

Genetic recombination, in the broadest sense, can be defined as any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules. All...

Cells possess a wealth of posttranscriptional control mechanisms that impact on every conceivable aspect of the life of an mRNA. These processes are intimately intertwined in an almost baroque manner, where promoter context influences the recruitment of...

Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal the unique structural features and biochemical functions of this protein. Several unique properties of p53 posed a challenge to understa- ing its normal function in...

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods...

Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism...

This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle...

This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids...

This comprehensive treatise on the reticuloendothelial system is a project jointly shared by individual members of the Reticuloendothelial (RE) Society and bio medical scientists in general who are interested in the intricate system of cells and...

Omnis cellula e cellula, "every cell from a cell," was dogma to the 19th century cellular physiologist and the cornerstone of Virchow's Cellular pathologie. "Spread out a cell into a layer and you will find that, in ceasing to be a cell,...

This volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to...

This manual consists of 30+ experiments that coincide and complement each of the 18 chapters in the Karp text (978-1-1182-0673-7.) There are three possible designs of the lab book, based on the instructor's needs. These designs focus on either...

Innovation is added value to a known process. Bioprinting: Techniques and Risks for Regenerative Medicine aims to stimulate a scientifically grounded, interdisciplinary, multiscale debate and exchange of ideas using the techniques described in the book...

Molecular Basis of Nutrition and Aging: A Volume in the Molecular Nutrition Series focuses on the nutritional issues associated with aging and the important metabolic consequences of diet, nutrition, and health. The book is subdivided into four parts...

DNA Structure and Function, a timely and comprehensive resource, is intended for any student or scientist interested in DNA structure and its biological implications. The book provides a simple yet comprehensive introduction to nearly all aspects of DNA...

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA...

In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics...

"Gene sharing" means that the different functions of a protein may share the same gene--that is, a protein produced by a gene evolved to fulfill a specialized function for one biological role may also perform alternate functions for other biological...

Selective Sweep deals with the theory and practice of detection of recent adaptive evolution at the genomic level from the patterns of DNA polymorphism. Recent advances in genomic sequencing provide the background for analysis of polymorphic sites in...

Landmark Experiments in Molecular Biology critically considers breakthrough experiments that have constituted major turning points in the birth and evolution of molecular biology. These experiments laid the foundations to molecular biology by uncovering...

While most books on dentistry describe the clinical and medical procedures involved, this book expands the field to examine the role of dentistry and teeth in everything from biology to biography. This book offers facts and figures regarding famous...

Molecular Tools and Infectious Disease Epidemiology examines the opportunities and methodologic challenges in the application of modern molecular genetic and biologic techniques to infectious disease epidemiology. The application of these techniques...

The study of G-quadruplexes has emerged in recent years as an important focus of research in nucleic acids. This is now a rapidly growing area, not least because of its potential as a novel approach to cancer therapeutics, and there is much current...

Progression of chronic diseases in general and chronic kidney disease in particular has been traditionally viewed in the light of various contributors to development of glomerulosclerosis and tubulointerstitial scarring culminating in renal fibrosis...

Molecular Aspects of Alcohol and Nutrition is a valuable resource for nutrition researchers and nutritionists who study or treat alcohol-related diseases. Experts from across the field of alcohol research explain how alcohol disrupts normal fat,...

Many human genetic diseases associated with blood, brain, colon, ear, eye, heart, kidney, liver, muscle, and pancreas are caused by mutations in mitochondrial DNA. Mutations in DNA can result in defects of the electron transport complexes, intermediates...

In this book emerging questions of current glutamate research are addressed and new pathways which glutamte research may take in the future discussed. Since glutamate antagonists are still the major hope for the therapy of neurodegenerative disorders in...

.For there to be progress in science, there must first be communication between experts of different disciplines. This is particularly true of modern biology which is becoming more and more of an interdisciplinary field. The present situation in cell...

The origin and function of normal monocytes and macrophages have been clearly defined by extensive investigations in human and in animal models. The central importance ofthis cell system for the biological defense mecha- nisms is well established:...

The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body. In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute...

Trinucleotide repeats are relatively common in the human genome. These simple repeats have received much attention since epoch-making discoveries were made that particular trinucleotide repeats are expanded in the causal genes of human hereditary...

Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip (R),...

This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of...

This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin...

When setting out to decide on the content of DNA Repair Protocols: Prokaryotic Systems, I was conscious of the need to portray the vast array of pathways and enzymatic activities that are part of the discipline of DNA repair. In addition to the classical...

Examines new research on the role of cholesterol in regulating ion channels and receptors and its effect on health Drawing together and analyzing all the latest research findings, this book explores the role of cholesterol in the regulation of ion...

The enormous advances made in molecular biology have allowed scientists to manipulate DNA with relative ease. This means that molecular biology has become a widely-used tool for answering scientific questions that may be quite unrelated to genetics and...

Systematics: A Course of Lectures is designed for use in an advanced undergraduate or introductory graduate level course in systematics and is meant to present core systematic concepts and literature. The book covers topics such as the history of...

This latest volume in the Harvey Lectures Series reflects "the evolution of physiology and physiological chemistry into biochemistry and the development of molecular biology from the roots of bacteriology and biochemistry" in the 20th and 21st...

Few scientists have the knowledge to perform the studies that are necessary to discover and characterize enzyme inhibitors, despite the vested interest the pharmaceutical industry has in this field. Beginning with the most basic principles pertaining to...

Cellular proteinases and their physiological role in normal and disease states have been the subject of great interest over recent decades. At present, specific protease inhibitors are exploited both as tools in unraveling the role of individual...